Fig. 1.

Mutation of OsMYB80 induced no-pollen male sterility in rice. (A–F) Whole plants, panicles, anthers and pollen grains stained with I₂-KI were compared between the WT and osmyb80 mutant. (G, H) The anthers and pollen grains from osmyb80 mutant complemented with the OsMYB80 gene. Scale bars: 10 cm in (A); 2 cm in (B); 1 mm in (C), (E) and (G); 100 μm in (D), (F) and (H). (I) Identification of the causal gene in HT5763 and H5524 mutant plants. The top panel showed the distribution of Euclidean distance scores (ED⁶) of single nucleotide polymorphisms (SNPs) on chromosomes of HT5763 and H5524. The red lines represent the Loess regression curves of ED⁶ values. The colored dots indicate the mutations along the chromosomes. Arrow shows the position of OsMYB80 gene on chromosome 4. Gene structure and mutation sites in the OsMYB80 gene are shown. Black boxes represent exons, gray boxes represent untranslated regions (UTRs) and the lines between boxes represent introns. The DNA fragment for gene complementation is indicated by the line.