Fig. 1.

Isolation and characterization of genotypes deficient in the expression of the NAD⁺ mitochondrial transporter (NDT2) in Arabidopsis thaliana mutant lines. (A) Schematic representation of the gene AtNDT2 (At1g25380) showing the T-DNA insertion site. The T-DNA insert, ∼4.5 kb, is not at scale. Boxes represent gene exons, black arrows on the T-DNA denote primer positions used for population screening and red arrows denote primers used for gene expression. The antisense construct includes the HYG, the 35S promoter, the gene NDT2 in antisense position and the 35S terminator. (B) Expression by the qRT-PCR analysis of NDT2 in flowers of the A. thaliana WT and mutant lines. Values are presented as mean � SE of determinations on six individual plants per line; asterisks indicate values that were determined by Student’s t-test to be significantly different (P < 0.05) from WT. (C) Phenotypic characterization of A. thaliana genotypes with the reduced expression of NDT2. The plants were 4 weeks old and grown in short-day conditions. HYG, hygromycin resistance gene.