Fig. 5.

Gene expression analysis and changes in the pyridine nucleotide levels in seedlings of NDT2 mutant lines and wild type (WT) plants. (A) Expression of NAD⁺ transporters NDT2, NDT1 and PXN genes in ndt2⁻:ndt2⁻ and WT. (B) Expression of NAD⁺ biosynthetic pathway genes: AO, quinolinate synthase (QS), QPRT, NaMNAT, NAD synthetase (NADS), PARP, nucleoside diphosphates linked to some moiety X (NUDIX), nicotinate phosphoribosyltransferase (NaPRT) and NIC in NDT2 mutant and WT seedlings. (C) Changes in the pyridine nucleotides in 4- and 8-day-old seedlings harvested in the middle of the light period, NAD⁺, NADH, NADH/NAD⁺, NADP⁺, NADPH, NADPH/NADP⁺. The values indicate means of four repeats � SE for gene expression and five replicates � SE for pyridine nucleotides (indicated by error bars). Asterisks indicate values that were determined by Student’s t-test to be significantly different (P < 0.05) from WT.