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. Author manuscript; available in PMC: 2020 Dec 26.
Published in final edited form as: J Med Chem. 2019 Dec 13;62(24):11437–11443. doi: 10.1021/acs.jmedchem.9b01589

Fig. 2.

Fig. 2

Characteristics of SCS-Ins in comparison with native Insulin. A) Circular dichroism spectra measured at 25 °C with a concentration of 0.2 mg/mL in pH 7.4 PBS. B) Human insulin signaling activation (Akt phosphorylation) assay. Data represent the average of 4 independent measurements and error bar represents SD. calc EC50 (95% CI): [Native Insulin = 1.91 (1.36–2.69) nM; SCS-Ins = 4.01 (2.91–5.53) nM]. C) Insulin tolerance test in mice. Data represent average of 4 male and female mice and error bar represents SD. D) Kinetic profiling of fibrillation, monitoring change in transmittance (i.e., increase in turbidity) at 1.0 mg/mL under continuous agitation at 37 °C in pH 7.4 PBS. E) Serum stability comparison at 37 °C at 0.1 mg/mL insulin concentration in human serum. Data represent 3 replicates at each time point and error bar represents SD. F) Thermal stability assay at 60 °C with 0.1 mg/mL insulin in pH 7.4 PBS. Data represent 3 replicates at each data point and error bar represents SD. 95% CI = 95% confidence intervals; SD = standard deviation; *p<0.05, Student t (unpaired) test.