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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Metab Eng. 2020 Mar 25;60:56–65. doi: 10.1016/j.ymben.2020.03.007

Fig. 5. Zn2+ dosage-dependent overexpression of the PsmtA::PCK::3×FLAG protein in strain SA590-PK-PCK.

Fig. 5.

Expression of the PsmtA::PCK::3×FLAG protein was induced for 6 h with different concentrations of Zn2+ and assessed by Western blot. Seven micrograms of cell extract was loaded in each lane, and equal loading was confirmed by Coomassie blue staining. Extracts from strain SA590-PK grown in LL were included as a negative control.