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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: FEBS Lett. 2020 Feb 6;594(9):1389–1402. doi: 10.1002/1873-3468.13740

Figure 3. MAG and CNS myelin increase Rap1GAP1 protein levels and suppress activation of Rap1.

Figure 3.

(A, B) Western blots of P5-6 CGN treated with MAG-Fc (20 μg/ml) or CNS myelin (20 μg/ml) for 20 minutes (n=8 for MAG-Fc, n=3 for myelin). Rap1GAP1 was quantified by normalizing Rap1GAP levels to their respective loading controls. Graphs depict average fold changes ± SEM. (*p < 0.05, paired one-tailed Student’s t-test). (C, D) Western blots of P5-6 CGN treated with MAG-Fc (20 μg/ml) or CNS myelin (20 μg/ml) for 20 minutes (n=3 for MAG-Fc, n=3 for myelin). Lysates were used for Rap1 activation assays detecting GTP-bound Rap1, and total Rap1 was assessed using input samples. For quantification, Rap1 GTP levels were normalized to the total Rap1 levels in the input lysate. Graphs depict average fold changes ± SEM (*p < 0.05, ***p < 0.001, paired one-tailed Student’s t-test).