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. 2020 May 12;9(5):46. doi: 10.1038/s41389-020-0230-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Induction of ACSS3 knockdown by doxycycline in BLCA cells. b Spheroid formation in BLCA cells stably transfected with control of ACSS3 shRNA with or without doxycycline administration under high serum or low serum culture. c Xenografts formed by BLCA cells stably transfected with control of ACSS3 shRNA with or without doxycycline administration under high serum or low serum culture. Right panels are measurements of tumor volumes at indicated time points.