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. 2020 May 12;11:2358. doi: 10.1038/s41467-020-16080-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Colocalization of microglia (Iba1, red) with Aβ (ThioS, green) and quantification in cortex of APP/PS1 injected with vehicle and APP/PS1 injected with N-AS mice. Scale bars, 20 μm; 3D reconstruction from confocal image stacks scale bars, 15 μm (n = 6 mice per group). b Left, representative photomicrograph of live slice section incubated with fluorescent beads (green) in WT, APP/PS1, and APP/PS1 injected with N-AS mice. Scale bar, 10 μm. White arrow point to phagocytic microglia with fluorescent beads. Right, quantification of the number of microglial phagocytes normalized to the total number of microglia (n = 5–6 mice per group). c Left, gating strategy for detection of CD45^hiCD11b⁺Gr-1^-F4/80⁺MHCII^hi (Pro-inflammatory macrophage) and of CD45^hiCD11b⁺Gr-1^-F4/80⁺CD206^hi (Anti-inflammatory macrophage) cells within brain cell populations. Right, graph displaying the calculated percentage of CD45^hiCD11b⁺Gr-1^-F4/80⁺MHCII^hi (Pro-inflammatory macrophage) and of CD45^hiCD11b⁺Gr-1^-F4/80⁺CD206^hi (Anti-inflammatory macrophage) cells in WT, APP/PS1, and APP/PS1 treated with N-AS mice brain (n = 6 mice per group). d Left, gating strategy for detection of CD45⁺CD11b⁺Ly6C^hiLy6G⁻ monocyte within brain cell populations. Right, graph displaying the calculated percentage of CD45⁺CD11b⁺Ly6C^hiLy6G⁻ monocyte in WT, APP/PS1, and APP/PS1 treated with N-AS mice brain (n = 6 mice per group). e Left, gating strategy for detection of CD45⁺CD11b⁺Gr-1⁺ neutrophil and CD45⁺Ly6G⁺Gr-1⁺ neutrophil within brain cell populations. Right, graph displaying the calculated percentage of CD45⁺CD11b⁺Gr-1⁺ neutrophil and CD45⁺Ly6G⁺Gr-1⁺ neutrophil in WT, APP/PS1, and APP/PS1 treated with N-AS mice brain (n = 6 mice per group). f Left, histograms are representative of CD4⁺ T cell proliferation at brain. Right, graph displaying the calculated percentage of CD4⁺ T cell in WT, APP/PS1, and APP/PS1 treated with N-AS mice brain (n = 6 mice per group). g Left, histograms are representative of B220⁺ B proliferation at brain. Right, graph displaying the calculated percentage of B220⁺ B cell in WT, APP/PS1, and APP/PS1 treated with N-AS mice brain (n = 6 mice per group).All data analysis was performed on 9-months-old mice. a–g One-way analysis of variance, Tukey’s post hoc test. All error bars indicate s.e.m. Source data are provided as a Source data file.