Figure 3.

Analysis of BiHR38, preBiHR38, BiEgr1, and BiEcR expression levels after seizure induction. Time-course of the expression after awakening from CO₂ anesthesia of BiHR38 (a), preBiHR38 (b), BiEgr1 (c), and BiEcR (d). The expression level of each gene was analyzed by qRT-PCR and normalized with that of BiEF1α. Magenta lines indicate the group anesthetized with CO₂ (“CO₂”), light blue dashed lines indicate the negative control (“NC”, continuously anesthetized with CO₂ for 120 min), and gray dotted lines indicate the positive control (“PC”, exposed just to air flow). All data indicate means ± SEM. Significant differences on the basis of Dunnett’s test after the ANOVA are indicated (*p < 0.05 for CO₂ group, ^‡p < 0.05 for PC group). Student’s t test and Welch’s t test revealed no significant difference between the NC group and the CO₂ 0-min group. Some errors were so low that it is difficult to see the error bars in the graph. n = 5 for each sample.