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. 2020 May 12;10:7887. doi: 10.1038/s41598-020-64701-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In situ hybridization of BiHR38, BiEcR, and BiEgr1 in forager brain sections. Expression of BiHR38 (a–d), BiEgr1 (e–g), and BiEcR (h–j) was analyzed by in situ hybridization. Sections of brain hemispheres hybridized with antisense (a,e,h) or sense probes (b,f,i) are shown. Panel (k) indicates schematic drawing of the brain hemisphere and panel (l) indicates the magnified view of the MB enclosed by the red square in panel (k). Panels (c,d,g,j) indicate magnified views of the MB area in panels (a,e,h), respectively. (a–c) Forager collected at 9:00 in the laboratory flight-cage; (d–j) forager collected as “Forager” in the greenhouse. Representative signals are indicated by red arrows. Processing-induced damage to the tissue is indicated by yellow arrows (e–g, respectively). The s (m,l)-KCs: small-type (middle-, large-) Kenyon cells, AL: antennal lobe, OL: optic lobe. Scale bars = 500 µm.