Table 4.

Gene expression of A. mellifera normalized using EF1α.

Mean ± SEM	MB
	6:30		8:30	9:00	9:30		12:30		22:30
	Nurse bee	Forager	Forager	Forager	Nurse bee	Forager	Nurse bee	Forager	Nurse bee	Forager
AmHR38	1 ± 0.23	2.17 ± 0.47	2.25 ± 0.42	1.91 ± 0.20	0.95 ± 0.08	2.36 ± 0.50	1.65 ± 0.55	8.13 ±1.13	3.73 ± 0.40	3.01 ± 0.52
preAmHR38	1 ± 0.23	1.71 ± 0.24	2.40 ± 0.37	5.54 ± 1.60	1.25 ± 0.20	5.41 ± 1.54	1.04 ± 0.07	5.48 ± 1.01	1.15 ± 0.39	1.16 ± 0.30
AmEgr1	1 ± 0.12	0.98 ± 0.25	1.40 ± 0.22	1.50 ± 0.25	0.95 ± 0.07	1.82 ± 0.32	0.90 ± 0.06	5.11 ± 1.03	1.27 ± 0.26	1.17 ± 0.56
AmEcR	1 ± 0.11	0.86 ± 0.13	0.86 ± 0.09	1.04 ± 0.12	0.83 ± 0.07	0.96 ± 0.11	0.93 ± 0.09	2.46 ± 0.54	0.97 ± 0.12	0.71 ± 0.06
Mean ± SEM	Other brain region
AmHR38	0.50 ± 0.16	0.92 ± 0.26	1.51 ± 0.31	1.50 ± 0.52	0.80 ± 0.16	1.75 ± 0.28	0.70 ± 0.15	3.17 ± 0.72	0.81 ± 0.13	0.70 ± 0.13
preAmHR38	0.26 ± 0.02	0.51 ± 0.07	3.17 ± 0.40	3.35 ± 1.18	0.45 ± 0.10	4.07 ± 0.76	0.29 ± 0.07	3.14 ± 0.78	0.24 ± 0.06	0.24 ± 0.08
AmEgr1	0.95 ± 0.06	1.34 ± 0.21	2.39 ± 0.32	2.29 ± 0.40	0.97 ± 0.11	2.38 ± 0.17	0.88 ± 0.06	3.04 ± 0.37	1.16 ± 0.15	1.13 ± 0.21
AmEcR	2.07 ± 0.26	3.15 ± 0.25	2.78 ± 0.11	2.87 ± 0.44	2.29 ± 0.23	2.40 ± 0.13	2.27 ± 0.14	3.03 ± 0.62	2.68 ± 0.13	2.60 ± 0.17

Relative gene expression in each brain tissue of workers normalized to AmEF1α in the A. mellifera sampling compared with the MBs of nurse bees collected at 6:30.