Fig. 4. Foxn1^CreROSA26^TdTOMATO as a model of thymic cooperative antigen transfer.

a Experimental design. b Flow cytometry heat-map analysis showing the intensity of TdTOM fluorescence among MACS TCRβ-depleted cells from the thymus of the Foxn1^CreROSA26^TdTOMATO mouse. c Representative flow cytometry plots comparing the frequency of TdTOM⁺CD11c⁺ cells in the thymic MACS-enriched CD11c⁺ cells between the WT (Foxn1^Cre) and Foxn1^CreROSA26^TdTOM mouse. Cells were pre-gated as live, singlets, and Gr-1^–. d Quantification of TdTOM⁺CD11c⁺ cells from c (mean ± SEM, n = 6 mice). Statistical analysis was performed by unpaired, two-tailed Student’s t-test, p < 0.0001 = ****. e Representative flow cytometry histograms showing the frequency of TdTOM⁺ cells among pDC, cDC1, and cDC2 (gated as in Supplementary Fig. 4a). Gray histograms = Foxn1^Cre (control) mice, red histograms = Foxn1^CreROSA26^TdTOM mice. f Quantification of frequencies of TdTOM⁺ DCs among the indicated DC subsets (mean ± SEM, n = 6 mice). g Representative images from the Imagestream analysis showing intracellular localization of transferred TdTOM in MHCII⁺CD11c⁺ DCs from the thymus of Foxn1^CreROSA26^TdTOMATO (n = 400 measured cells).