Fig. 6. Development of thymic Tregs is impaired in MyD88^ΔTECs mice.

a Representative flow cytometry plots (left plot) and their quantification (right plot) comparing the frequencies of main thymic T cell populations between MyD88^fl/fl and MyD88^ΔTECs mice (mean ± SEM, n = 14 mice). b Representative flow cytometry plots comparing the frequencies of CD4⁺CD25⁺Foxp3⁺ thymic Tregs between MyD88^fl/fl and MyD88^ΔTECs mice. c Quantification of frequencies from b (mean ± SEM, n = 14 mice). d Representative flow cytometry histograms showing the expression of CD73 by CD4⁺CD25⁺Foxp3⁺ thymic Tregs (gated as in b). e Quantification of the total numbers of CD73^– and CD73⁺ thymic Tregs from d (mean ± SEM, n = 7 mice). f Quantification of the frequencies of thymic Tregs from CpG ODN or PBS intrathymically stimulated (7 days) MyD88^fl/fl or MyD88^ΔTECs mice (mean ± SEM, n = 4 for MyD88^ΔTECs and n = 9 for MyD88^fl/fl mice). g Quantification of the total numbers of CD73^– and CD73⁺ thymic Tregs from CpG ODN or PBS intrathymically stimulated (7 days) WT (C57Bl/6J) mice (mean ± SEM, n = 6 for ODN⁺ and n = 7 for ODN^– mice). h Quantification of frequencies of thymic Tregs from CpG ODN or PBS intrathymically stimulated (7 days) H2-Ab1^fl/fl or H2-Ab1^fl/flItgax^Cre (H2-Ab1^ΔDCs) mice (mean ± SEM, n = 6 for H2-Ab1^fl/fl and ODN⁺ H2-Ab1^ΔDCs and n = 7 for ODN^– H2-Ab1^ΔDCs mice). i Quantification of the total numbers of CD73^– and CD73⁺ thymic Tregs from CpG ODN or PBS intrathymically stimulated (7 days) H2-Ab1^ΔDCs mice (mean ± SEM, n = 6 for ODN⁺ and n = 7 for ODN^– mice). Statistical analysis in a, c, e–i was performed by unpaired, two-tailed Student’s t-test, p ≤ 0.05 = *, p ≤ 0.01 = **, p ≤ 0.001*** p < 0.0001****, ns not significant.