Figure 1.

Isolated MPs improve thrombin generation in all HA plasma models and in PNP as detected by the CAT assay. (a) Thrombin generation in the severe HA plasma model with different concentrations of MPs (MP-0, 2, 3 and 7: 0, 2, 3 and 7 × 10⁴ MPs/µL plasma), in the presence (solid lines) and absence (dashed lines) of PPP-Reagent LOW (CAT reagent). The inset shows thrombin generation curves (with an adjusted y-axis scale) in the absence of CAT reagent. (b) Peak thrombin value in the severe HA plasma model. (c–e) Thrombin generation in other plasma models with MPs (2 × 10⁴ MPs/µL plasma) in the presence (solid lines) and absence (dashed line) of CAT reagent: (c) moderate HA (2.5% FVIII); (d) mild HA (20% FVIII), and (e) PNP (100% FVIII). (f) Peak thrombin value in the moderate, mild HA plasma models and in PNP. In all plasma models, without MPs and without CAT reagent, the thrombin generation curves were flat at baseline level. Data shown are mean ± SEM values, n = 9 replicates.