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. 2020 May 12;10:7871. doi: 10.1038/s41598-020-64686-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Standard confocal microscopy showed that MPs increased fibrin clot density in the severe HA plasma model. (a) Fibrin clot structure in the severe HA plasma model and PNP control, with (MPs 2) and without (MPs 0) addition of MPs (2 × 10⁴ MPs/µL plasma). Images shown are representative slices from optical sections (scale bar = 25 µm); (b) Fibrin clot density analyzed by using Fiji software. The clot samples were all prepared in the presence of OHP reagent. Data are means ± SEM, n = 3 fibrin clots (8 optical stacks/clot). ****p < 0.0001 compared to severe HA model without addition of MPs.