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. 2020 May 12;10:7871. doi: 10.1038/s41598-020-64686-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PMPs incorporated in the fibrin network detected by STED. (a) PMPs attached to a fibrin fiber; green: fiber (Alexa fluor 594), red: PMPs (Aberrior STAR 635); (b) The corresponding intensity profile along the line shown in (a), intensity normalized on the basis of the minimum intensity value in each channel; (c,d) Different typical patterns of the location of PMPs in the fibrin network: (c) PMPs located at the cross point of two fibers; (d) Fiber turned direction near the attached PMPs; (e) PMPs embedded in a mesh of fibrin fibers; (f) PMPs act as a bridge connecting two fibers. Images are all from the severe HA plasma model with addition of MPs. Representative images from two independent experiments are shown; duplicate samples/experiment. Bar = 2 µm.