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. 2020 May 12;11:2369. doi: 10.1038/s41467-020-15733-8

Fig. 3. MLL-AF9 leukemia is delayed in Mbnl1 knockout mice.

Fig. 3

a Scheme of mouse leukemia model. Wild type (WT) or knockout (KO) lineage negative bone marrow cells were transduced with an MLL-AF9 GFP-expressing retrovirus, followed by serial replating in semisolid medium to select for MLL-AF9 cells. Selected cells were then transplanted into irradiated primary recipient mice (n = 10 per group). b Graph represents percent of leukemia-free survival of primary recipient mice. ***p = 0.0003 by two-sided Log-rank (Mantel-Cox) test. c Distressed primary recipient mice 12 weeks after transplantation were sacrificed and splenocytes were harvested and plated for CFU assay. After 7 days in semi-solid culture spleen cells were injected into secondary irradiated recipients (n = 5 mice per group). Graph depicts percent of leukemia-free survival. **p = 0.0088 by two-sided Log-rank (Mantel-Cox) test.