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. 2020 May 12;11:2369. doi: 10.1038/s41467-020-15733-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a RT-PCR analysis of regions of select MBNL1 regulated genes (INF2, ATP2A1, and CD47) across a panel of MLL-rearranged and wild-type leukemia cells. b RT-PCR analysis of INF2, ATP2A1, and CD47 transcripts demonstrating change in splicing pattern in MOLM13 cells after MBNL1 knockdown. Band percentages (relative to each individual lane) are noted. c Alternative splicing patterns of MBNL1 target genes are shown after 48 h of Compound 1 treatment at the indicated dosages. Relative proportion of each AS feature indicated in decimal adjacent to corresponding band. All gels are representative images of two biological replicates.