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Main mechanisms of endogenous H2S production in mammalian cells. Endogenous H2S can be produced mainly through three pathways. Among which, l-cysteine directly generates H2S through the catalysis of CBS or CSE. H2S is also biosynthesized by the synergistic effects of CAT and 3-MST. Ultimately, H2S is metabolized in mitochondria in the form of thiosulfate or sulfate.
