Fig. 1.
Effect of different concentrations of a MgSO4, b betaine, c temperature, and d duration on the detection of cardamom vein clearing virus (CdVCV) by reverse transcriptase loop-mediated isothermal amplification (RT-LAMP). Detection was done under UV light and agarose gel electrophoresis. a MgSO4. Lanes 0, 2, 4, 6, 8, and 10, are loaded with products of RT-LAMP carried out using different concentrations of MgSO4. b Betaine. Lanes 0.4, 0.6, 0.8, 1.0, 1.2, and 1.4 are loaded with products of RT-LAMP carried out using different concentrations of betaine. c Temperature. Lanes 58, 62, 65, and 68 are loaded with products of RT-LAMP carried out at different temperatures. d Duration. Lanes 15, 30, 45, 60, and 75 are loaded with products of RT-LAMP carried out over different durations. Lane M shows molecular size marker and Lane WC is water control