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. 2020 May 6;11:782. doi: 10.3389/fimmu.2020.00782

TABLE 3.

Combination of antibodies used in flow cytometry experiments.

A. Combinations of 4 antibodies
Antibody 1 Antibody 2 Antibody 3 Antibody 4
CD4 FITC CD95 PE CD25 PE-Cy7 CD8 APC
CD4 FITC CCR4 PE CD25 PE-Cy7 CD8 APC
CD4 FITC CD25 PE CXCR5 biotin + Streptavidin PE-CY7 CD8 APC
CXCR3 FITC CD4 PE CD25 PE-Cy7 CD8 APC
CCR7 FITC CD4 PE CD25 PE-Cy7 CD8 APC
B. Combinations of 5 or 6 antibodies
Antibody 1 Antibody 2 Antibody 3 Antibody 4 Antibody 5 Antibody 6
CD4 APC CD8 PE-Cy7 CD25 PE CD127 BV 421 CD45RA FITC CD31 PerCp-eF 710
CD4 PerCp-Cy5.5 CD8 PE-Cy7 CD25 PE CD127 BV 421 CD45RA FITC FOXP3 eF660
CD4 PerCp-Cy5.5 CD8 PE-Cy7 CD25 PE CD127 BV 421 CXCR3 FITC
CD4 PerCp-Cy5.5 CD8 PE-Cy7 CD25 PE CD127 BV 421 CXCR5 Alexa Fluor 488
CD4 PerCp-Cy5.5 CD8 PE-Cy7 CD25 PE CD127 BV 421 CCR4 FITC CCR7 APC
CD4 PerCp-Cy5.5 CD8 PE-Cy7 CD25 PE CD127 BV 421 CD95 APC