TABLE 3.
Kinetic parameters of OB-1 and DooKu variants.
| Substrate | Kinetic constant | OB-1 | DooKu |
| ABTS | Km (mM) | 0.007 ± 0.0006 | 0.009 ± 0.0009 |
| kcat (s–1) | 690.0 ± 20.2 | 1,328.8 ± 42.3 | |
| kcat/Km (mM–1 s–1) | 106,073.8 | 140,000.8 | |
| DMP | Km (mM) | 0.15 ± 0.01 | 0.23 ± 0.01 |
| kcat (s–1) | 565.0 ± 13 | 1,129.0 ± 18.4 | |
| kcat/Km (mM–1 s–1) | 3,842.6 | 5,561.8 | |
| Sinapic Acid | Km (mM) | 0.32 ± 0.02 | 0.45 ± 0.06 |
| kcat (s–1) | 741.0 ± 14.1 | 1,433.06 ± 65.2 | |
| kcat/Km (mM–1 s–1) | 2,286.4 | 3,184.6 | |
| K4Mo(CN)8 | Km (mM) | 0.81 ± 0.2 | 0.81 ± 0.1 |
| kcat (s–1) | 220.5 ± 12.1 | 252.4 ± 11.5 | |
| kcat/Km (mM–1 s–1) | 271.6 | 313.2 | |
| Guaiacol | Km (mM) | 2.6 ± 0.4 | 3.4 ± 0.4 |
| kcat (s–1) | 117.4 ± 6.2 | 171.0 ± 7.0 | |
| kcat/Km (mM–1 s–1) | 44.6 | 50.3 |
Kinetic constants were estimated in 100 mM phosphate buffer pH 4.0 and 25°C. All reactions were performed in triplicate.