Figure 1.

Phosphorylation of histone cluster 1 H1 family member c (H1.2) is regulated by the metastasis-associated 1 (MTA1). (A,B) Normal liver THLE-2 cells were transfected with pEGFP-N1 (empty vector) or pEGFP-MTA1-expressing plasmids. The H1.2 at threonine-146 residue (H1.2^T146ph) levels were then determined using Western blotting. Shown are representative blots (A) and quantification of three independent experiments (B). **P < 0.01, t-test. (C,D) SNU449 and HUH6 liver cancer cells were transfected with pEGFP-N1 (empty vector) or pEGFP-MTA1-expressing plasmids. The H1.2^T146ph levels were then determined using Western blotting. Shown are representative blots (C) and quantification of three independent experiments (D). **P < 0.01, t-test.