Figure 2.

Histone cluster 1 H1 family member c at threonine-146 residue (H1.2^T146ph) is involved in the pro-oncogenic function of MTA1. (A) HuH6 cells were transfected with pEGFP-N1, pEGFP-H1.2, pEGFP-MTA1, and pEGFP-H1.2^T146E plasmids indicated as GFP, H1.2, MTA1, and H1.2^T146E (0.5, 1, and 2 μg, respectively), and relative expression of MTA1 and H1.2 were determined by Western blotting. Shown are representative blots from three independent experiments. (B) Cell viability was detected in the transfected HuH6 cells. **P < 0.01, t-test. (C,D) Colony formation experiment was performed using soft agar. Representative images (C) and quantification of numbers of colonies (D) in the transfected HuH6 cells. **P < 0.01, t-test. (E,F) Transwell assays were performed in the transfected HuH6 cells. Shown are representative images (E) and quantification (F) of the migrating cells (F).**P < 0.01, t-test.