Figure 3.

Transcription of metastasis-associated 1 (MTA1)-targeted genes were partially rescued by increased expression of histone cluster 1 H1 family member c at threonine-146 residue (H1.2^T146ph). (A) Expressions of genes were tested using quantitative reverse transcription PCR (qRT-PCR) in HuH6 cells transfected with pEGFP-N1 + H1.2, pEGFP-MTA1 + H1.2, pEGFP-MTA1 + H1.2^T146E plasmids (indicated as GFP, +H1.2, MTA1 + H1.2, and MTA1 + H1.2^T146E, respectively). **P < 0.01, t-test. (B) Interaction of H1.2^T146ph with the indicated genes was determined by chromatin immunoprecipitation (ChIP) assays in HuH6 cells transfected with pEGFP-N1 + H1.2 and pEGFP-MTA1 + H1.2 plasmids (indicated as GFP + H1.2 and MTA1 + H1.2, respectively). **P < 0.01, t-test.