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. 2020 May 6;10:567. doi: 10.3389/fonc.2020.00567

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Copyright © 2020 Li, Zhong, Zang and Tian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Metastasis-associated 1 (MTA1) overexpression was associated with DNA protein kinase (DNA-PK) degradation. (A) Semiquantitative PCR was performed to assess DNA-PK messenger RNA (mRNA) expression in HuH6 cells transfected with pEGFP-MTA1 or pEGFP-N1. (B) Whole cell extracts from HuH6 cells that were transfected with DNA-PK-HA, pEGFP-MTA1, or pEGFP-N1 plasmids (left panel) and pEGFP-MTA1 or pEGFP-N1 plasmids were analyzed using Western blotting. (C) Western blotting results were shown in MG132 or dimethyl sulfoxide (DMSO)-treated HuH6 cells after transfecting with indicated plasmids. (D) Whole cell extracts from HuH6 cells that were untreated (the samples were collected at 24, 48, and 51 h following transfection, left panel) or treated with MG132 (MG132 was added at 48 h following transfection, and the samples were collected at 24, 48, 51, and 54 h following transfection; right panel) were analyzed using Western blotting.