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. 2020 May 6;11:411. doi: 10.3389/fphys.2020.00411

FIGURE 5.

FIGURE 5

AgRP neuron inhibition abolishes acute exercise induced food intake. (A) Top, targeting scheme for hM4Di-mCherry. Bottom, localization of hM4Di-mCherry with the anatomical location of NPY/AgRP neurons in the ARC. (B) Immunofluorescence and quantification of mCherry positive and cfos-expressing cells in the ARC from AAV CNO and AAV Saline groups immediately after a bout of acute exercise. (C) Cumulative food intake over an 8-hour period immediately after the exercise or sedentary conditions. # indicates significance between Exercise CNO and Sedentary CNO groups (p < 0.05); * indicates a significant difference between Exercise AAV Saline and Exercise AAV CNO groups (p < 0.05) (N = 6 male mice per group). (I.P. CNO and saline injections of 0.3 mg/kg of body weight were applied 30 min prior to exercise). Bar graphs show Mean + SEM. 3V: third ventricle, scale bars represent 50 μm.