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. 2020 May 6;11:411. doi: 10.3389/fphys.2020.00411

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Copyright © 2020 Bunner, Landry, Laing, Li, Rao, Yuan and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Acute exercise induces PVN^SIM1 expressing neuronal activation independent of ARC^AgRP/NPY neurons. (A) Immunofluorescence of SIM1-positive and cfos-expressing cells in the PVN from the sedentary, exercise, AAV CNO, and AAV Saline groups immediately after a bout of acute exercise. 3V = third ventricle; scale bars represent 50 μM. (B) Quantification of SIM1-positive cells in the PVN among the four groups. (C) Quantification of cfos colocalization with SIM1-positive cells in the PVN from the sedentary, exercise, AAV CNO, and AAV Saline groups immediately after a bout of acute exercise. 3V: third ventricle, scale bars represent 50 μm. Bar graphs show Mean + SEM. (N = 6 from 3 male mice per group), * indicates p < 0.05 vs. sedentary group.