TABLE 3.

Summarized biosynthesis processes highlighted in roots of maize plants treated with a consortium of arbuscular mycorrhizal fungi (AMF), Trichoderma koningii TK7, and rhizosphere bacteria or with a consortium of Trichoderma koningii TK7 and rhizosphere bacteria (Tricho) plus a protein hydrolyzate-based biostimulant (PH), or with a protein hydrolyzate-based biostimulant (PH).

	AMF + Trichoderma			PH			PH + Trichoderma
	Number of compounds	Average FC	Sum FC	Number of compounds	Average FC	Sum FC	Number of compounds	Average FC	Sum FC
Amino acid	18	–3.2	–58.5	19	4.0	75.3	19	4.4	82.7
Nucleosides and Nucleotides	7	2.5	17.8	7	4.5	31.6	7	7.2	50.6
Fatty acid and Lipid	29	3.1	90.0	29	7.8	225.6	29	6.5	189.5
Amines and Polyamines	4	2.7	10.8	4	–2.2	–8.7	4	–2.2	–8.7
Carbohydrates	4	2.6	10.4	5	10.8	54.0	5	12.4	61.9
Secondary Metabolites	224	–2.8	–621.6	242	3.3	809.2	244	3.0	741.1
Cofactors, Prosthetic Groups, Electron Carriers	43	–0.2	–7.4	44	3.2	141.1	44	2.9	126.5
Hormones	31	–2.0	–62.4	35	5.7	197.9	35	4.8	166.7
Cell structures	6	–7.2	–43.1	6	4.3	26.1	6	5.2	31.3
Metabolic Regulators	3	0.5	1.5	3	–0.7	–2.1	3	–0.7	–2.1
Other biosynthesis	19	–5.3	–100.7	20	–0.7	–13.1	20	–0.7	–14.9

The metabolomic dataset produced through UHPLC-ESI/QTOF-MS was subjected to a Volcano Plot analysis (P < 0.01, fold-change > 1.2) and differential metabolites were loaded into PlantCyc Pathway Tool (https://www.plantcyc.org/). The average and summed Log fold-changes (Log FC) values, together with the number of compounds involved, is provided for each biosynthetic pathway and for each treatment.