Figure 4.

miR-449a Suppresses Cell Proliferation and Colony Formation Capacity of LCa Cells

(A) Basal miR-449a expression levels in two LCa cell lines (Hep-2 and HNO210) and HaCaT cells. U6 snRNA was used as a housekeeping gene. (B) miR-449a expression in transfected Hep-2 and HNO210 cells with either miR-449a mimic or inhibitor, or the corresponding NC. U6 snRNA was used as a housekeeping gene. (C and D) Cell proliferation was determined after the transfection of either miR-449a mimic or inhibitor, or the corresponding NC in (C) Hep-2 and (D) HNO210 cells. (E) Colony formation was determined in Hep-2 cells transfected with either miR-449a mimic or inhibitor, or the corresponding NC. Each sample was run in triplicate. Error bars show mean ± SD. The t test was used for the calculation of p values. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.