Table 1.

Composition and protocol of use of the materials tested.

Material	Composition	Application protocol
Adhesive system: Single Bond Universal (3M ESPE, St Paul, USA)	MDP phosphate monomer, HEMA, dimethacrylate resins, Vitrebond copolymer, filler, ethanol, water, initiators, silane	The adhesive was actively applied with a microbrush for 20 s, followed by air blast for 5 s with distance of 10 cm, and cure for 10 s (LED light, 1200 mW/cm2 - Radii-cal, Australia).
Nd: YAG laser (Pulse Master 600 IQ, Texas, USA)	Wavelength of 1.096 μm, power of 60 mJ	It was used a quartz optical fiber with a diameter of 320 μm, 1 mm from the surface, with irradiance for 60 s. The pulse length was 0.1 ms, and energy density was 74.64 J/cm2
Calcium Phosphate: TeethMate Desensitizer (Kuraray Noritake Dental Inc.)	Dicalcium phosphate anhydrate, Tetracalcium phosphate, water, preservative	Active application over occlusal dentin surface with a microbrush for 30 s, followed by water/air spray and dry with absorbent paper. This application was performed previously adhesive and/or laser irradiance.