Fig. 1.

Quantification of exosomes released from ARPE-19 cells. (A) NanoSight nanoparticle tracking analysis of vesicles released form ARPE-19 cells. The histograms show the particle size distributions, and the red area ± standard error of the mean from five samples per group. (B) Immunoblot analysis of CD63, HSP70, and GM130 in cell lysates and purified exosomes. ARPE-19 cells were treated with TNF-α and/or TGF-β2 for 3 days. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)