Fig. 2.

PBMCsec prevents differentiation-associated marker expression and DC cytokine secretion. Representative dot plots of (a) CD1a and (c) CD11c expressions of MoDC differentiated in the presence of vehicle medium and PBMCsec on day 7. Numbers in (a) and (c) indicate frequency of cells. N = 5 and n = 3 donors in (a) and (c), respectively. Statistical analysis of (b) CD1a⁺ and (d) CD11c⁺ cells in MoDC-vehicle and MoDC-PBMCsec. * indicates p<.05 PBMCsec versus vehicle. N = 5 donors and n = 3 donors in (b) and (d), respectively. Ordinary one-way ANOVA and Dunnett's multiple comparisons of all groups versus vehicle were performed. (e) CD1a expression dynamics in the course of DC differentiation with PBMCsec addition at indicated time points. N = 3 donors. (f) Flow cytometric assessment of DC maturation marker expressions in LPS-challenged MoDC-vehicle and MoDC-PBMCsec. Representative histograms of n = 3 donors are shown. (g) Quantification of secreted IL-12p70 by ELISA. N = 3 donors. Asterisk denotes p<.05 LPS-challenged MoDC-PBMCsec versus LPS-treated MoDC-vehicle. Ordinary one-way ANOVA was performed and LPS-treated groups were compared by Sidak's multiple comparisons.