Fig. 6.

PBMCsec and lipids functionally impair MoDCs. Amounts of (a) IL-12p70 and (b) TNF-α released by LPS-treated MoDC-PBMCsec and MoDC-lipids. * indicates p<.05 versus vehicle. N = 3 donors per group. Ordinary one-way ANOVA and Dunnett's multiple comparisons of all groups versus vehicle were performed. (c) Intensity of cell proliferation dye staining reflecting non-proliferating and proliferating cell populations in MLR. Numbers indicate frequencies per gate. Representative histograms of biological triplicates are shown. (d) Quantification of proliferating and non-proliferating cells. N = 3 donors per group. Concentrations of (e) secreted IL-12p70 and (f) secreted TNF-α during MLR with MoDC-medium, MoDC-PBMCsec, and MoDC-lipids. * denotes p<.05 versus vehicle. N = 3 donors per group. Ordinary one-way ANOVA and Dunnett's multiple comparisons of all groups versus vehicle were performed. (g) Dextran uptake of MoDC differentiated with control medium, PBMCsec, or lipids. Numbers indicate frequencies of cells per gate. One representative histogram of biological triplicates is shown. Secretion of (h) IFNγ and (i) IL-13 during co-culture of naïve T cell with MoDC differentiated in the presence of medium vehicle, PBMCsec, or lipids. * indicates p<.05 versus vehicle. N = 3 donors per group. Ordinary one-way ANOVA and Dunnett's multiple comparisons of all groups versus vehicle were performed.