Figure 1.

Cell viability and viral replication in HAdV-infected MT-2 cells. (a to d) MT-2 cells five days post infection with HAdV-19. Cell death was observed in a dose-dependent manner. (a) mock-infected, (b) 10² TCID₅₀, (c) 10³ TCID₅₀, (d) 10⁴ TCID₅₀. (e) inhibition of formazan formation in virus-infected and mock-infected MT-2 cells. Formazan formation by mock-infected, and HAdV-19-infected MT-2 cells was monitored for seven days after infection. (f) The qPCR detection of HAdV-19 in culture medium. MT-2 cells were infected with HAdV-19 for four or seven days before qPCR detection of HAdV-19 DNA in culture supernatant. HAdV DNA levels are shown as fold change for each day after infection compared with the amount at 0 days post-infection. After four and seven days of infection, the amount of viral DNA increased by more than 50 and 150 times compared with the initial amount of viral DNA, respectively.