FIG 7.

Syk-deficient neutrophils are unable for form NETs. (A) Cas9 and two Syk KO neutrophil cell lines were coincubated with Candida species at an MOI of 10. Where indicated, 2 μM Syk inhibitor PRT was added. Arbitrary units (AU) represent Sytox fluorescence corresponding with NET formation. **, P ≤ 0.01; ****, P ≤ 0.0001; ns, not significant. (B) NETs formed by Cas9 and Syk KO neutrophils were stained with Sytox green and anti-Cit-H3 histone-specific antibody and visualized by fluorescence microscopy. Scale bar, 25 μm. (C) Scanning electron microscopy (SEM) images of neutrophil cell lines coincubated with Candida species. Scale bars, 10 μm for the ×2,000 image and 1 μm for the ×10,000 image. Data represent results from a minimum of three independent experiments.