Fig. 5.

AA treatment activated the AMPK and inhibited PI3K/AKT signaling pathway. The cells were treated with or without AA (5 μM) for 3 days. a Representative western blot of p-AMPK, AMPK, p-PI3K, PI3K, p-AKT, and AKT. GAPDH was served as a loading control. b Relative protein expression of p-AMPK/AMPK, p-PI3K/PI3K, and p-AKT/AKT. Unpaired t test, *P < 0.05, ***P < 0.001. Each experiment was repeated three times (p-AMPK, phosphorylated AMP-activated protein kinase; AMPK, AMP-activated protein kinase; p-PI3K, phosphorylated phosphoinositide-3 kinase; PI3K, phosphoinositide-3 kinase; p-AKT, phosphorylated protein kinase B; AKT, protein kinase B)