Table 1.
Table of species, concentration and source of the antibodies used for immunostaining
| CD4 | T helper cells | Mouse | 1:2000 | AbD Serotec |
|---|---|---|---|---|
| CD8 | Cytotoxic T cells | Mouse | 1:2000 | AbD Serotec |
| CD79a1 | B cells | Mouse | 1:2000 | Thermoscientific |
| IFNγ | Cytokine | Mouse | 1:100 | Santa Cruz |
| TNF | Cytokine | Goat | 1:100 | R&D Systems |
| Iba1 | Microglia/Macrophages | Rabbit | 1:2000 | Wako, Japan |
| NeuN | Neuronal Nuclei | Mouse | 1:1000 | Chemicon |
| MOG | Myelin/oligodendrocytes | Mouse | 1:50 | R. Reynolds |
| CNPase | Myelin/oligodendrocytes | Mouse | 1:1000 | Millipore |
| NFH | 200 kDa Neurofilament protein | Mouse | 1:1000 | Chemicon |
| Cl-Casp3 | Apoptotic cells | Rabbit | 1:1000 | Cell Signalling |
| MLKL | TNF signalling |
Rabbit Mouse |
1:500 1:250 |
Cell signalling Santa Cruz |
| pMLKL | Necroptotic cells | Rabbit | 1:500 | Abcam |
| RIPK3 | TNF signalling | Rabbit | 1:300 | Sigma |
| pRIPK3 | Necroptotic cells | Rabbit | 1:500 | Abcam |
| Olig2 | Oligodendrocytes | Rabbit | 1:500 | Abcam |