Table 2.
PCR array data showing the differential expression of cell death pathway genes in IFA aloned and MOG + IFA immunised animals injected with cytokine viral vectors compared to GFP vector injected control animals at 28 days post injection. Genes highlighted in bold and italics showed a greater than 2-fold significant increase
| Gene | Fold change (compared to GFP control) | |||||
|---|---|---|---|---|---|---|
| MOG | IFA | |||||
| Fold Change | 95% Cl | p-value | Fold Change | 95% Cl | p-value | |
| Aifm1 | 1.78 | (1.28, 2.28) | 0.017 | 2.28 | (1.45, 3.10) | 0.033 |
| Ar | 3.32 | (1.47, 5.18) | 0.026 | 3.97 | (2.09, 5.85) | 0.008 |
| Atp6v1g2 | 2.32 | (1.19, 3.45) | 0.035 | 2.81 | (1.92, 3.71) | 0.002 |
| Bax | 1.45 | (0.95, 1.96) | 0.091 | 1.53 | (1.02, 2.04) | 0.054 |
| Bid | 1.94 | (0.94, 2.95) | 0.118 | 1.93 | (1.53, 2.33) | 0.002 |
| Birc3 | 4.33 | (2.12, 6.54) | 0.034 | 4.86 | (3.48, 6.23)3.48, 6.23)(1.47, 2.62) | 0.0001 |
| Bmf | 2.23 | (0.87, 3.59) | 0.115 | 2.05 | (1.47, 2.62) | 0.003 |
| Capn2 | 2.00 | (1.31, 2.69) | 0.053 | 2.29 | (1.52, 3.05) | 0.013 |
| Capn3 | 2.80 | (1.53, 4.06) | 0.042 | 2.20 | (1.42, 2.98) | 0.007 |
| Capn5 | 1.97 | (1.66, 2.29) | 0.001 | 1.97 | (1.53, 2.41) | 0.005 |
| Capn7 | 1.80 | (1.04, 2.55) | 0.068 | 2.07 | (1.57, 2.57) | 0.003 |
| Capns1 | 2.53 | (1.44, 3.63) | 0.037 | 2.81 | (1.69, 3.92) | 0.021 |
| Cd40 | 4.78 | (2.30, 7.25) | 0.047 | 3.91 | (2.59, 5.23) | 0.002 |
| Cetn1 | 2.36 | (1.14, 3.59) | 0.033 | 2.09 | (1.04, 3.14) | 0.049 |
| Cyba | 5.43 | (2.06, 8.79) | 0.107 | 4.05 | (3.39, 4.71) | 0.0001 |
| Cybb | 17.03 | (3.33, 30.73) | 0.059 | 11.17 | (5.71, 16.64) | 0.003 |
| Cyld | 3.00 | (2.06, 3.93) | 0.007 | 2.23 | (1.06, 3.39) | 0.070 |
| Defb1 | 5.87 | (3.01, 8.73) | 0.015 | 3.12 | (0.27, 5.97) | 0.192 |
| Dpys14 | 1.79 | (1.32, 2.26) | 0.014 | 2.08 | (1.37, 2.79) | 0.027 |
| Fadd | 1.71 | (1.06, 2.35) | 0.041 | 2.14 | (1.19, 3.09) | 0.066 |
| Fas | 3.49 | (1.45, 5.54) | 0.107 | 2.24 | (1.77, 2.71) | 0.001 |
| Faslg | 1.61 | (0.88, 2.34) | 0.167 | 2.10 | (1.38, 2.81) | 0.014 |
| Foxi1 | 22.90 | (0.00001, 65.05) | 0.091 | 23.61 | (0.00001, 72.42) | 0.323 |
| Glud1 | 2.06 | (1.35, 2.78) | 0.052 | 2.14 | (1.36, 2.93) | 0.021 |
| Glul | 2.29 | (1.39, 3.18) | 0.021 | 2.50 | (0.95, 4.06) | 0.088 |
| Grb2 | 2.65 | (1.95, 3.34) | 0.004 | 2.69 | (1.83, 3.55) | 0.007 |
| Hspbap1 | 2.08 | (1.62, 2.55) | 0.005 | 2.22 | (1.45, 2.98) | 0.022 |
| Kcnip1 | 1.77 | (1.03, 2.51) | 0.050 | 2.40 | (1.43, 3.36) | 0.019 |
| Madd | 1.63 | (1.03, 2.22) | 0.046 | 2.27 | (1.45, 3.10) | 0.022 |
| Myd88 | 2.91 | (1.56, 4.27) | 0.088 | 2.52 | (1.99, 3.04) | 0.001 |
| Nfkb1 | 2.34 | (1.69, 3.00) | 0.004 | 2.24 | (1.53, 2.95) | 0.007 |
| Ngf | 2.04 | (1.36, 2.27) | 0.008 | 2.37 | (1.39, 3.35) | 0.012 |
| Ngfr | 3.32 | (1.68, 4.96) | 0.024 | 2.59 | (1.28, 3.90) | 0.031 |
| Nox1 | 3.44 | (1.77, 5.11) | 0.008 | 4.23 | (2.53, 5.94) | 0.001 |
| Parp1 | 1.85 | (1.06, 2.64) | 0.078 | 1.98 | (1.48, 2.48) | 0.004 |
| Parp2 | 1.25 | (0.90, 1.60) | 0.200 | 1.36 | (1.15, 1.57) | 0.008 |
| Ppid | 2.09 | (1.38, 2.80) | 0.036 | 2.23 | (1.59, 2.87) | 0.005 |
| Pygl | 4.06 | (1.84, 6.28) | 0.048 | 3.31 | (1.83, 4.79) | 0.010 |
| Rab25 | 2.55 | (1.27, 3.83) | 0.027 | 2.42 | (1.45, 3.38) | 0.005 |
| Dennd4a | 1.96 | (1.25, 2.67) | 0.041 | 2.13 | (1.57, 2.70) | 0.003 |
| Ripk1 | 1.80 | (1.29, 2.31) | 0.022 | 1.94 | (1.26, 2.62) | 0.027 |
| Ripk2 | 1.64 | (1.17, 2.10) | 0.056 | 1.88 | (1.43, 2.34) | 0.010 |
| Ripk3 | 4.16 | (1.83, 6.48) | 0.049 | 2.71 | (2.00, 3.42) | 0.0001 |
| Sp1 | 1.92 | (1.31, 2.53) | 0.036 | 2.03 | (1.46, 2.61) | 0.012 |
| Spata2 | 2.36 | (1.89, 2.82) | 0.0001 | 2.66 | (1.44, 3.88) | 0.038 |
| Tmem123 | 2.85 | (1.60, 4.11) | 0.065 | 2.36 | (1.98, 2.75) | 0.000 |
| Tmem57 | 2.63 | (1.12, 4.14) | 0.055 | 2.04 | (1.11, 2.97) | 0.018 |
| Tnf | 4.81 | (1.92, 7.71) | 0.049 | 3.38 | (1.69, 5.06) | 0.039 |
| Tnfrsf10b | 2.19 | (1.09, 3.29) | 0.037 | 2.23 | (1.32, 3.14) | 0.003 |
| Tnfrsf14 | 51.83 | (0.00001, 120.6) | 0.028 | 34.17 | (0.00001, 79.86) | 0.030 |
| Tnfrsf17 | 2.10 | (1.74, 2.47) | 0.001 | 2.15 | (1.58, 2.73) | 0.013 |
| Tnfrsf1a | 2.62 | (1.80, 3.44) | 0.018 | 2.21 | (1.55, 2.86) | 0.009 |
| Tnfrsf1b | 6.02 | (3.58, 8.46) | 0.014 | 4.12 | (3.36, 4.89) | 0.0001 |
| Tnfrsf25 | 1.91 | (1.01, 2.80) | 0.023 | 2.26 | (0.78, 3.74) | 0.130 |
| Tnfrsf8 | 4.24 | (2.84, 5.63) | 0.003 | 4.14 | (3.14, 5.15) | 0.0001 |
| Tnfrsf10 | 2.63 | (1.57, 3.70) | 0.029 | 2.59 | (1.82, 3.37) | 0.001 |
| Tradd | 2.11 | (1.71, 2.52) | 0.002 | 2.10 | (1.46, 2.74) | 0.014 |
| Traf2 | 2.30 | (1.60, 2.99) | 0.020 | 2.38 | (1.64, 3.11) | 0.009 |
| Txnl4b | 2.91 | (1.49, 4.32) | 0.114 | 2.23 | (1.62, 2.84) | 0.005 |