Table 1.
Validation of TaqMan assays with laboratory isolates
| Strain | Assay | Replicates | Plasmepsin Est. FC | MDR-1 Est. FC | ||
|---|---|---|---|---|---|---|
| Avg | SD (range) | Avg | SD (range) | |||
| 3D7 | plasmepsin 2 | 8 | 1.00 | 0.02 (0.97–1.04) | – | – |
| Dd2 | plasmepsin 2 | 8 | 1.06 | 0.03 (1.03–1.12) | – | – |
| PH1097-C | plasmepsin 2 | 6 | 1.06 | 0.02 (1.04–1.09) | – | – |
| PH1265-C | plasmepsin 2 | 6 | 1.89 | 0.02 (1.85–1.93) | – | – |
| PH1310-C | plasmepsin 2 | 6 | 1.06 | 0.03 (1.01–1.13) | – | – |
| PH1387-C | plasmepsin 2 | 6 | 1.93 | 0.09 (1.8–2.04) | – | – |
| 3D7 | plasmepsin 3 | 8 | 1.02 | 0.03 (0.96–1.08) | – | – |
| Dd2 | plasmepsin 3 | 8 | 1.09 | 0.03 (1.05–1.15) | – | – |
| PH1097-C | plasmepsin 3 | 6 | 1.06 | 0.02 (1.03–1.09) | – | – |
| PH1265-C | plasmepsin 3 | 6 | 2.05 | 0.06 (1.93–2.14) | – | – |
| PH1310-C | plasmepsin 3 | 6 | 1.00 | 0.03 (1.02–1.09) | – | – |
| PH1387-C | plasmepsin 3 | 6 | 2.29 | 0.07 (2.19–2.38) | – | – |
| 3D7 | plasmepsin 2/pfmdr1 | 8 | 1.01 | 0.02 (0.97–1.04) | 1.00 | 0.02 (0.96–1.02) |
| Dd2 | plasmepsin 2/pfmdr1 | 8 | 1.10 | 0.04 (1.04–1.17) | 1.83 | 0.06 (1.77–1.92) |
| PH1097-C | plasmepsin 2/pfmdr1 | 6 | 1.07 | 0.05 (1–1.13) | 1.06 | 0.05 (1.01–1.11) |
| PH1265-C | plasmepsin 2/pfmdr1 | 6 | 1.80 | 0.03 (1.75–1.84) | 0.96 | 0.02 (0.93–0.99) |
| PH1310-C | plasmepsin 2/pfmdr1 | 6 | 1.02 | 0.02 (1–1.06) | 1.01 | 0.02 (0.98–1.05) |
| PH1387-C | plasmepsin 2/pfmdr1 | 6 | 1.89 | 0.05 (1.84–2) | 0.93 | 0.03 (0.91–0.99) |