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. Author manuscript; available in PMC: 2020 May 13.
Published in final edited form as: Obesity (Silver Spring). 2016 Mar 29;24(6):1244–1256. doi: 10.1002/oby.21463

Figure 1.

Figure 1

Zn delayed obesity-induced renal hypertrophy and dysfunction. Obesity was induced by feeding HFD with different concentrations of Zn (10/30/90 mg Zn per 4057 kcal) for 3 and 6 M. (A) Liver Zn concentration was measured by atomic absorption spectroscopy as a reference of animal tissue Zn level at 6 M. (B) Ratio of kidney weight to tibia length was measured and calculated, respectively. (C) Renal function was evaluated by calculating the UACR of spot urine samples at 3 and 6 M. (D) Kidney triglyceride concentration was assayed both in 3 and 6 M. (E) Kidney lipid infiltration and accumulation were examined by Oil-Red-O staining (200×, scale bar 200 μm) and semiquantitative analysis for lipid accumulation (red area) per image was counted in 10 vision fields across the kidney. Data are presented as the mean ± S.D. (n≥5). *, P < 0.05 vs. corresponding ND/N.Zn; #, P < 0.05 vs. corresponding HFD/N.Zn.