Skip to main content
. Author manuscript; available in PMC: 2020 May 13.
Published in final edited form as: Obesity (Silver Spring). 2016 Mar 29;24(6):1244–1256. doi: 10.1002/oby.21463

Figure 4.

Figure 4

Zn inhibited the kidney inflammation response and the activation of P38 MAPK induced by obesity. (A–D) Renal mRNA level of MCP-1, IL-1β, IL-6, and TNF-α was detected by RT-PCR both in 3 and 6 M as the evaluation of renal inflammation response. (E) TNF-α protein expression level in kidney was examined by Western blot. (F) IHC staining of p-P38 MAPK of 3 and 6 M (400×, scale bar 100 μm). (G) Western blotting of p-P38 MAPK and total P38 MAPK after feeding the animals with different concentration of Zn. This was followed by the ratio of p-P38 to total P38 and IHC staining positive area of p-P38 to evaluate the activation of the P38 MAPK pathway. Data are presented as the mean ± S.D. after normalizing to ND/N.Zn (n≥5). *, P < 0.05 vs. corresponding ND/N.Zn; #, P < 0.05 vs. corresponding HFD/N.Zn.