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. 2020 Jan 29;21(5):1875–1885. doi: 10.1021/acs.biomac.9b01745

Figure 7.

Figure 7

HepG2 cells inside CNF-alginate-CLPs based printed scaffolds. (a) Microscopy images of CNF-CLP25 scaffolds after 1 day of incubation. Phase-contrast image, focused on the edge of the scaffold, displays the poor light permeability of the scaffolds. However, Calcein AM and PI staining enabled monitoring of cellular growth inside the scaffolds. Scale bar is 200 μm. (b) 3D reconstruction from Calcein AM and PI staining Z-stacks (5 μm spacing) of a CNF-CLP25 scaffold after 1 day of incubation, which displays the attachment of HepG2 cells mostly in a horizontal direction. (c) 3D reconstruction from Calcein AM and PI staining Z-stacks (5 μm spacing) of a CNF-CLP25 scaffold after 5 days of incubation, which displays the growth of HepG2 cells in a horizontal and vertical direction.