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. Author manuscript; available in PMC: 2020 May 13.
Published in final edited form as: Biochemistry. 2018 Jun 7;57(26):3976–3986. doi: 10.1021/acs.biochem.8b00511

Figure 4.

Figure 4.

Effect of sodium, chloride, and potassium extracellular concentration on ZIP2 transport activity. (A) Changes in fluorescence intensity of Calcium 5 dye in response to Cd2+ perfusion (1 μM) measured in HEK293 cells transiently transfected with DsRed-Express2 and ZIP2 DNA constructs in KB buffer (pH 6.5) in which extracellular Na+ or Cl was replaced with equimolar NMDG, choline+, and K+ or gluconate salts, respectively. Data from two independent experiments were normalized to the mean ZIP2 activity at pH 6.5 and are represented as means ± SD (n = 8–12). (B) Uptake of 63Zn2+ in the presence of 100 μM ZnCl2 by ZIP2- and H2O-injected X. laevis oocytes measured in ND96 (pH 6.0) in which extracellular Na+ was replaced with equimolar choline+ or K+. Data from two different batches of oocytes were normalized to the mean Zn2+ uptake by ZIP2 at pH 6.0 (403 ± 62 pmol oocyte–1 min–1) and are represented as means ± SD (6–12 oocytes). P values establish statistical differences between hZIP2-mediated Zn2+ uptake at pH 6.0 and the indicated experimental conditions.