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. Author manuscript; available in PMC: 2020 May 13.
Published in final edited form as: Cell Rep. 2019 Jul 23;28(4):1090–1102.e3. doi: 10.1016/j.celrep.2019.06.068

Figure 2. Mec1 S1964 and T1902 mutants do not alter intrinsic Mec1 activity and DNA repair.

Figure 2.

(A) γ-H2AX levels in Mec1 point mutants in a tel1Δ background 3h after the induction of an irreparable DSB, normalized to WT. Error bars represent SEM.

(B) Sensitivity of Mec1 T1902 and S1964 mutants to DNA damaging reagents HU, phleomycin and UV.

(C) The schematic of YMV80 recovery system. The viability (left) and Rad53 hyperphosphorylation (right) of Mec1 T1902 and S1964 mutants after recovery in YMV80. **p=0.0078, compared to WT. Error bars represent SEM.

(D) Schematic of the gene conversion YJK17 system, and the viability of mec1-S1964A mutant in YJK17. p=0.059. Error bars represent SEM.