KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Rad53 | AbCam | EL7.E1 |
| Mouse monoclonal anti-HA | AbCam | 12CA5 |
| Mouse monoclonal anti-myc | AbCam | 9E10 |
| Mouse monoclonal anti-Pgk1 | AbCam | 22C5D8 |
| Anti-phospho-H2-S129 | AbCam | ab15083 |
| Rabbit polyclonal anti-Rad9 | (Usui et al. 2009) | N/A |
| Rabbit polyclonal anti-Mec1 | (Hustedt et al. 2015) | N/A |
| Rabbit polyclonal anti-phosho-Mec1-S1964 | This paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Hydroxyurea (HU) | Sigma-Aldrich | H8627 |
| Phleomycin | InvivoGen | ant-ph-1 |
| Cycloheximide | Sigma-Aldrich | C7698-5G |
| Indole-3-acetic acid | Sigma-Aldrich | I3750-25G-A |
| Halt Protease Inhibitor | ThermoFisher | PI78430 |
| Protein-A agarose beads | Sigma-Aldrich | 1719408 |
| Protein-G agarose beads | Sigma-Aldrich | 11719416001 |
| Experimental Models: Organisms/Strains | ||
| S. cerevisiae: Strain background S228c. Strains are listed in SI Table 3. | This paper | N/A |
| Oligonucleotides | ||
| Oligonucleotides used as repair templates for Cas9-directed mutagenesis are listed in SI Table 2. | This paper | N/A |
| Recombinant DNA | ||
| Plasmids used in this study are listed in SI Table 1. | This paper | N/A |
| Software and Algorithms | ||
| Prism 7.00 | GraphPad Software, Inc. | |
| Image Lab | Bio-Rad | |
| FiJi | ||