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. Author manuscript; available in PMC: 2020 May 13.
Published in final edited form as: Cell Rep. 2020 Mar 17;30(11):3932–3947.e6. doi: 10.1016/j.celrep.2020.02.091

Figure 6. FLIM Data Validating scRNA-Seq-Predicted Metabolic Heterogeneity in WO Skin.

Figure 6.

(A) Gene scoring analysis of all four UW basal subclusters using an oxidative phosphorylation signature. p values are from two-sided Wilcoxon rank-sum tests.

(B) Gene scoring analysis of all four WO basal subclusters. p values are from two-sided Wilcoxon rank-sum tests.

(C) Sketch diagram of wound epithelium showing the areas probed with FLIM.

(D) A representative image of wound epidermal cells indicated by GFP expression.

(E) Representative images of NADH signal and NADH lifetime signals.

(F) A representative phasor plot with cell phasor fingerprint, which is a representation of the fluorescence lifetime decay of all cells in the region of interest (ROI) after fast Fourier transformation.

(G) Violin plot incorporating all cells and their corresponding free/bound NADH ratios from four biological replicates (156 cells from the outside region, 127 cells from the adjacent region, and 231 cells from the neo-epidermis).

(H) Quantification of average free/bound NADH ratios from multiple cells from the four biological replicates of various regions of the wound. For statistical analysis we used an unpaired two-tailed Student’s t test. Error bars represent mean ± SEM.