Table 1.
Summary of data on symbiosis and germination in 18 populations of autumn bluegrass, Poa autumnalis, with latitude and longitude for each population. Populations in bold were additionally surveyed for prevalence in seedlings produced by field-collected seeds from the surveyed adult plants.
| Site | Endophyte taxa | Collection dates | Latitude | Longitude | Adult prevalence (%) | N (plants) | Stromata adults (%) | N (seeds) | Seed prevalence (%) | N (germ.) | Seed germination (%) | N (seedlings) | Seedling prevalence (%) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ANF-1 | 1 & 2 | 2009-04-11 2009-05-08 | 31°02′52″ | −94°23′16″ | 100.0 | 30 | 0.0 | 400 | 23.2 | 73 | 70.9 | ||
| ANF-2 | 1 | 2009-05-08 | 31°05′01″ | −94°19′21″ | 97.1 | 35 | 0.0 | 438 | 47.0 | 192 | 96.3 | ||
| ANF-3 | 1 & 2 | 2009-04-11 2009-05-08 | 31°05′13″ | −94°19′15″ | 96.7 | 43 | 20.0 | 1351 | 33.9 | 480 | 96.9 | ||
| ANF-5 | 1 & 2 | 2006-04-12 | 31°05′23″ | −94°19′21″ | 100.0 | 14 | 0.0 | 21 | 90.5 | ||||
| ANF-6 | 1 | 2006-04-12 | 31°02′52″ | −94°23′14″ | 100.0 | 12 | 0.0 | 22 | 100.0 | ||||
| BNP-1 | 1 & 2 | 2006-04-16 | 30°34′24″ | −94°38′32″ | 93.3 | 15 | 0.0 | 9 | 100.0 | ||||
| BNP-2 | 1 & 2 | 2006-04-17 | 30°29′15″ | −94°50′27″ | 100.0 | 14 | 0.0 | 20 | 100.0 | ||||
| BNP-3 | 1 | 2006-04-17 | 30°23′00″ | −94°51′00″ | 100.0 | 22 | 0.0 | 20 | 100.0 | ||||
| DNF-1 | 1 | 2009-05-04 | 31°27′56″ | −95°07′44″ | 93.3 | 30 | 0.0 | 705 | 56.0 | 259 | 99.4 | ||
| DNF-2 | 1 | 2006-05-16 | 31°27′46″ | −95°07’44’’ | 100.0 | 14 | 0.0 | 19 | 78.9 | ||||
| LOC-1 | 1 & 2 | 2006-04-26 | 31°38′57″ | −94°46′37″ | 100.0 | 20 | 0.0 | 23 | 100.0 | ||||
| SFA-1 | 1 | 2009-04-30 | 31°29′44″ | −94°45′38″ | 100.0 | 30 | 0.0 | 970 | 89.0 | 593 | 98.8 | ||
| SFA-2 | 1 | 2006-04-30 | 31°30′07″ | −94°47′18″ | 90.0 | 20 | 0.0 | 71 | 94.4 | ||||
| SFA-3* | 1 | 2007-05-02 | 31°29′44″ | −94°45′39″ | 100.0 | 25 | 0.0 | n.t. | n.t. | 98 | 95.9 | ||
| SNF-1 | 1 & 2 | 2009-04-23 | 30°30′30″ | −95°05′22″ | 100.0 | 30 | 0.0 | 780 | 35.3 | 151 | 81.6 | ||
| SNF-2 | 1 & 2 | 2006-04-12 | 30°30′30″ | −95°05′22″ | 94.1 | 17 | 0.0 | 16 | 68.8 | ||||
| SNF-3 | 1 | 2006-04-12 | 30°30′48″ | −95°07′23″ | 91.7 | 12 | 0.0 | 18 | 61.1 | ||||
| TWR-1 | 2 | 2006-04-17 | 30°21′58″ | −94°51′43″ | 91.7 | 12 | 0.0 | 21 | 100.0 |
*The SFA-3 population was used in the field experiment; the other populations were used to survey natural endophyte prevalence. Collection dates are given in ISO 8601 format. Prevalence (in adults, seeds or seedlings) is the mean percentage of individuals with the endophyte. N = sample size. n.t. = not tested.
Endophyte taxa were distinguished by PCR, taxon 1 = PauTG-1 (hybrid), taxon 2 (E. typhina subsp. poae, non-hybrid), 1 & 2 = a mixture of both PauTG-1 and E. typhina subsp. poae.