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. 2020 Mar 21;125(6):905–923. doi: 10.1093/aob/mcaa022

Fig. 4.

Fig. 4.

Immunolocalization of trans-zeatin riboside in the 2-week-old nodules of pea SGE wild type and corresponding mutants, general view. (A–C) SGE. (D–F) sym33-3. (G–I) sym40. (J–L) sym26. (A, D, G, J) Merge of differential interference contrast (DIC) and magenta channel. (B, E, H, K) Merge of green and magenta channels. A single optical section is presented: trans-zeatin riboside in green and DNA (bacteria and nuclei) in magenta. (C, F, I, L) The heatmap shows colour-coded fluorescence signal intensities for the green signal channel; the quantification scale is the same for all images. PI, propidium iodide; AF488, Alexa Fluor 488. I, meristem zone; II, infection zone; II', infection thread propagation zone; III, nitrogen fixation zone; III', zone corresponding to the nitrogen fixation zone of wild-type nodules. Scale bars are 100 µm.