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. 2020 Mar 21;125(6):905–923. doi: 10.1093/aob/mcaa022

Fig. 7.

Fig. 7.

Immunolocalization of trans-zeatin riboside in 2-week-old nodules of pea mutants sym33-3 (A–F) and sym40 (G–L). (A–C) Colonized cell with a ‘locked’ infection thread. (D–F) Colonized cell with an infection droplet. (G–I) Recently infected cell with an infection droplet. (J–L) Colonized cell with a hypertrophied infection droplet. (A, D, G, J) Merge of differential interference contrast (DIC) and magenta channel. (B, E, H, K) Merge of green and magenta channels. A single optical section is presented: trans-zeatin riboside in green and DNA (bacteria and nuclei) in magenta. (C, F, I, L) The heatmap shows colour-coded fluorescence signal intensities for the green signal channel; the quantification scale is the same for all images. PI, propidium iodide; AF488, Alexa Fluor 488. n, nucleus; arrows indicate infection threads; arrowheads indicate infection droplets. Scale bars are 10 µm.